Product description

Cell Counting Kit-8, abbreviated as CCK-8, is a rapid and highly sensitive assay for cell proliferation and cytotoxicity based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium monosodium salt), which is an upgraded version of MTT. WST-8 is an upgraded product of MTT, the working principle is: in the presence of electronically coupled reagents, it can be reduced by the dehydrogenase enzyme in the mitochondria to generate a highly water-soluble orange-yellow dirty product (formazan). The shade of color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The OD value is measured at 450 nm using an enzyme marker, indirectly reflecting the number of viable cells.CCK-8 method has a wide range of applications, such as drug screening, cell proliferation assay, cytotoxicity assay, tumor sensitivity assay, and the assessment of biological factor activity.

 

Components

Components No.	C331301E	C331301S	C331301M	C331301L	C331301T
Size	100 T	500 T	1000 T	3×1000 T	10×1000 T

 

Shipping and Storage

Ice packs shipping. 2~8℃ dry and lightproof storage, valid for one year.-20℃ dry and lightproof storage, valid for two years.