Product description

DMEM/F-12 Reduced Serum Medium is based on the classic DMEM/F-12 with the addition of ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin, and trace elements, which can be used to maintain cell growth in a low serum state for serum-reduced mammalian cell culture. It requires the addition of 1~5% fetal bovine serum and 4 mM L-glutamine or alanyl-glutamine prior to use. Due to the different serum dependence of different cell types, it is recommended to optimize for different cells to determine the final serum concentration. Compared to classic DMEM/F-12, the use of this formulation reduces the amount of serum used by 50~90% without affecting the cell growth rate and morphology, thus reducing the fluctuation of culture conditions and prolonging the use of serum from the same batch.

This product contains: Phenol Red.

This product does not contain: L-Glutamine, HEPES.

 

Components

Components No.	C231114E
Size	500 mL

 

Shipping and Storage

Ice packs shipping. 2~8℃ storage, valid for one year.