Product description

Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor and is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor IL-6R binding site, and two gp130 binding sites. IL-6 is a pleiotropic, alpha-helical, 22~28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a multifunctional cytokine that not only affects the immune system but also acts on various biological systems and physiological events in various organs, while also inducing the growth of bone marrow and plasma cell tumors.

The Arcegen Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of mouse interleukin-6 (Mouse IL-6) in serum and plasma. Specific antibodies against mouse interleukin-6 are pre-coated on a high-affinity enzyme immunoassay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, the mouse interleukin-6 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated for binding. After washing, enzyme conjugate (Streptavidin-HRP) is added and incubated for binding. After washing, a color substrate TMB is added for color development in the dark. The intensity of the color reaction is proportional to the concentration of mouse interleukin-6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm).

 

Specifications

Catalog Number	P162003S/P162003E
Specifications	48 T/96 T
Detection Range	15.63~1000 pg/mL
Detection Method	ELISA
Species Detected	Mouse
Detection Time	4.5 hours
Sensitivity	9.37 pg/mL
Dilution Linearity	74~107%
Recovery	77~111%
Intra-assay Variation	3.1%
Inter-assay Variation	5.7%

 

Components

Component Number	Component Name	Storage Temperature	P162003S	P162003E
P162003-A	ELISA Plate	2~8℃	48 T	96 T
P162003-B	Standard	2~8℃	1 tube	2 tubes
P162003-C	Detection Antibody	2~8℃	120 μL	240 μL
P162003-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162003-E	5×Dilution Buffer	2~8℃	8 mL	15 mL
P162003-F	20×Wash Buffer	2~8℃	25 mL	50 mL
P162003-G	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162003-H	Stop Solution	Room Temperature	5 mL	10 mL
P162003-I	Plate Sealant Film	Room Temperature	3 pieces	5 pieces

 

Shipping and Storage

Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After Initial Use

Material Name	Storage Conditions
Enzyme Plate	Unused strips can be returned to aluminum foil pouch, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection Antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme Conjugate
5×Dilution Buffer	Store at 2~8°C for 1 month, avoid contamination
20×Wash Solution
Substrate Solution	Store at 2~8°C for 1 month, protect from light.
Stop Solution	Can be stored at room temperature
Sealing Film