Product description

Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Human IL-15 in serum, plasma, and cell culture supernatants. Specific anti-IL-15 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-15 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Interleukin-15 (IL-15), a widely expressed 14 kDa cytokine, plays a crucial role in numerous immune-related diseases. It shares approximately 97% and 73% sequence identity with IL-15 from primates and rodents, respectively. IL-15 is secreted by monocytes/macrophages (and some other cells), especially macrophages following viral infection. With similar biological functions to IL-2, IL-15 stimulates T cell activation and proliferation by sharing the γc receptor subunit with IL-2. The roles of IL-15 in autoimmune processes (such as rheumatoid arthritis) and malignancies (such as adult T cell leukemia) underscore its significance as a key immunoregulatory molecule, garnering considerable attention from researchers.

 

Specifications

Item Number	P162018S / P162018E
Specification	48 T / 96 T
Detection Range	3.9-250 pg/mL
Detection Method	Sandwich ELISA
Detection Species	Human
Detection Time	4.5 hours
Sensitivity	1.58 pg/mL
Dilution Linearity	83 - 124%
Recovery Rate	82 - 116%
Intra-assay Variability	3.2%
Inter-assay Variability	5.8%

 

Components

Component Number	Component Name	Storage Temperature	P162018S	P162018 T
P162018-A	ELISA Plate	2~8℃	48 T	96 T
P162018-B	Standard	2~8℃	1 tube	2 tubes
P162018-C	Detection Antibody	2~8℃	120 μL	240 μL
P162018-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162018-E	5x Dilution Buffer	2~8℃	8 mL	15 mL
P162018-F	20x Wash Buffer	2~8℃	25 mL	50 mL
P162018-G	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162018-H	Stop Solution	Room Temperature	5 mL	10 mL
P162018-I	Plate Sealers	Room Temperature	3 pieces	5 pieces

 

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After First Use

Material Name	Storage Conditions
Plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme conjugate
5×Dilution Buffer	Store at 2~8°C for 1 month, avoiding contamination.
20×Wash Buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate Sealers