Product description

Human Fibroblast Growth Factor 10 (FGF-10), also known as Keratinocyte Growth Factor 2 (KGF-2), belongs to the FGF family and is a approximately 20 kDa protein with a region rich in serine near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is considered important during embryonic development and also plays a significant role in adipogenesis. FGF-10 is expressed in the pre-second heart field and, together with Fibroblast Growth Factor 8 (FGF-8), promotes the proliferation of cardiac progenitor cells forming the arterial pole of the heart.

The Arcegen Human FGF-10 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of FGF-10 in serum, plasma, and cell culture supernatants. Specific anti-FGF-10 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, FGF-10 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of FGF-10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm).

 

Specifications

Item Number	P162002E / P162002S
Specification	48 T / 96 T
Detection Range	1~64 ng/mL
Detection Method	Sandwich ELISA
Detection Time	4.5 hours
Sensitivity	0.69 ng/mL
Dilution Linearity	77~122%
Recovery Rate	77~121%
Intra-assay Variability	5.7%
Inter-assay Variability	9.2%

 

Components

Component Number	Component Name	Storage Temperature	P162002E	P162002S
P162002-A	ELISA Plate	2~8℃	48 T	96 T
P162002-B	Standard	2~8℃	1 tube	2 tubes
P162002-C	Detection Antibody	2~8℃	60 μL	120 μL
P162002-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162002-E	Sample Dilution Buffer	2~8℃	8 mL	15 mL
P162002-F	Antibody/Enzyme Dilution Buffer	2~8℃	15 mL	30 mL
P162002-G	20x Wash Buffer	2~8℃	25 mL	50 mL
P162002-H	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162002-I	Stop Solution	Room Temperature	5 mL	10 mL
P162002-J	Plate Sealant Film	Room Temperature	3 pieces	5 pieces

 

Shipping and Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After Initial Use

Material Name	Storage Conditions
ELISA plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard sample	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detecting antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme conjugate
Sample diluent	Store at 2~8°C for 1 month, avoiding contamination.
20×Wash solution
Antibody/enzyme diluent
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate seal film