Product description

Tumor necrosis factor-alpha (TNF-α), also known as cachexin or TNFSF1A, is a multifunctional cytokine that plays a central role in inflammation, apoptosis, and immune system development. It is produced by various immune cells, epithelial cells, endothelial cells, and tumor cells. TNF-α exerts its effects through receptors TNFR1 and TNFR2, activating signaling pathways involving caspase 8, transcription factor NF-κB, and kinase JNK.

Human TNF-α shares 79% amino acid homology with mouse TNF-α, suggesting that its biological functions may not exhibit significant species specificity. TNF-α has been shown to confer resistance against certain types of infections while paradoxically inducing inflammation in pathological processes. It may also affect glucose uptake and insulin resistance. Additionally, TNF-α plays a critical role in tumor proliferation, migration, invasion, and angiogenesis.

The Arcegen Mouse TNF-α ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit designed to quantitatively measure mouse TNF-α in serum and plasma samples. The assay utilizes high-affinity anti-mouse TNF-α antibodies pre-coated onto an ELISA plate. Standard samples and test samples are added to the plate wells and incubated, allowing mouse TNF-α present in the samples to bind to the solid-phase antibodies. After washing to remove unbound substances, a detection antibody is added for incubation, followed by addition of enzyme conjugate (Streptavidin-HRP). After washing again, a colorimetric substrate (TMB) is added for color development, and the intensity of the color reaction is proportional to the concentration of mouse TNF-α in the samples. The reaction is terminated, and absorbance is measured at 450 nm wavelength (with a reference wavelength of 570-630 nm).

 

Specifications

Item Number	P162006S / P162006E
Specification	48 T / 96 T
Detection Range	31.25-2000 pg/mL
Detection Method	Sandwich ELISA
Species Detected	Mouse
Detection Time	4.5 hours
Sensitivity	7.82 pg/mL
Dilution Linearity	84 - 124%
Recovery Rate	81 - 119%
Intra-assay Variability	3.8%
Inter-assay Variability	5.3%

 

Components

Component Number	Component Names	Storage Temperature	P162006S	P162006E
P162006-A	ELISA plate	2~8℃	48 T	96 T
P162006-B	Standard sample	2~8℃	1 tube	2 tubes
P162006-C	Detection antibody	2~8℃	120 μL	240 μL
P162006-D	Enzyme conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162006-E	5× dilution buffer	2~8℃	8 mL	15 mL
P162006-F	20× wash buffer	2~8℃	25 mL	50 mL
P162006-G	Substrate solution	2~8℃(Avoid Light)	8 mL	15 mL
P162006-H	Stop solution	Room Temperature	5 mL	10 mL
P162006-I	Plate sealing film	Room Temperature	3 pieces	5 pieces

 

Storage

The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After Initial Use

Component Names	Storage Conditions
ELISA plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard sample	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
Enzyme conjugate
5× dilution buffer	Store at 2~8°C for 1 month, avoiding contamination.
20× wash buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate sealing film