Product description

Human Bone Morphogenetic Protein-7 (Human BMP-7) is a member of the TGF-β superfamily and is considered one of the most potent synthetic factors for chondrocytes. The growth factor domain of human BMP-7 shares 98% amino acid sequence identity with mouse and rat BMP-7. BMP-7 acts in various organ systems. It promotes new bone formation and the development of renal units, inhibits differentiation of prostate epithelium, and antagonizes epithelial-mesenchymal transition. Bone Morphogenetic Protein (BMP) signaling regulates a series of cellular processes and plays an important role in early embryonic patterning. It is known that BMP-7 expression has a protective effect on renal injury during diabetic nephropathy and disappears in the early stages of diabetic nephropathy progression. Potential clinical applications of BMP-7 include its neuroprotective effects in stroke animal models.

The Arcegen Human BMP-7 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring BMP-7 in serum, plasma, and cell culture supernatants. Specific anti-BMP-7 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. BMP-7 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of BMP-7 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm).

 

Specifications

Item Number	P162014S / P162014E
Specification	48 T / 96 T
Detection Range	62.5-4000 pg/mL
Detection Method	Sandwich ELISA
Detection Time	4.5 hours
Sensitivity	44.6 pg/mL
Dilution Linearity	86 - 127%
Recovery Rate	81 - 126%
Intra-assay Variability	3.3%
Inter-assay Variability	4.2%

 

Components

Component Number	Component Name	Storage Temperature	P162014S	P162014E
P162014-A	ELISA Plate	2~8℃	48 T	96 T
P162014-B	Standard	2~8℃	1 tube	2 tubes
P162014-C	Detection Antibody	2~8℃	60 μL	120 μL
P162014-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162014-E	Sample Dilution Buffer	2~8℃	8 mL	15 mL
P162014-F	Antibody/Enzyme Dilution Buffer	2~8℃	15 mL	30 mL
P162014-G	20x Wash Buffer	2~8℃	25 mL	50 mL
P162014-H	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162014-I	Stop Solution	Room Temperature	5 mL	10 mL
P162014-J	Plate Sealant Film	Room Temperature	3 pieces	5 pieces

 

Storage

The kit can be stored at 2~8℃ or according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded, and they should not be reused. The shelf life is 1 year.

Table 1.Reagent Storage Table After First Use

Component Name	Storage Conditions
ELISA Plate	Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
Detection Antibody	Use within 48 hours after dilution,store at 2~8°C to avoid contamination.
Enzyme Conjugate
Sample Dilution Buffer	Store at 2~8°C for 1 month,avoiding contamination.
Antibody/Enzyme Dilution Buffer
20x Wash Buffer
Substrate Solution	Store at 2~8°C for 1 month,avoiding light exposure.
Stop Solution	Can be stored at room temperature.
Plate Sealant Film