Product description

Mouse interferon-gamma (Mouse IFN-γ) is a soluble homodimeric cytokine and the sole member of the type II interferon family. It is mainly secreted by natural killer (NK) cells and natural killer T (NKT) cells, playing a crucial role in immune function. Interferon-gamma (IFN-γ) is essential for innate and adaptive immunity against viral, bacterial, and parasitic infections. It acts as a vital activator of macrophages and induces the expression of major histocompatibility complex class II (MHC II). Aberrant expression of IFN-γ is associated with many autoimmune and inflammatory diseases. Besides directly inhibiting viral replication, IFN-γ is important for immune stimulation and regulation. It can effectively inhibit cell proliferation and is used clinically to treat various conditions such as primary thrombocythemia, chronic myeloid leukemia, polycythemia vera, and primary myelofibrosis.

The Arcegen Mouse IFN-γ ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit used for quantitative measurement of mouse interferon-gamma (Mouse IFN-γ) in serum and plasma. Specific anti-mouse interferon-gamma antibodies are precoated on a high-affinity enzyme-linked immunosorbent assay (ELISA) plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, mouse interferon-gamma present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation, followed by washing. Streptavidin-horseradish peroxidase (HRP) conjugate is then added for incubation. After another washing step, a colorimetric substrateTMB is added for color development. The intensity of the color reaction is proportional to the concentration of mouse interferon-gamma in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength range of 570 - 630 nm).

 

Specifications

Item Number	P162007S / P162007E
Specification	48 T / 96 T
Detection Range	31.25-2000 pg/mL
Detection Method	Sandwich ELISA
Detection Time	4.5 hours
Sensitivity	5.65 pg/mL
Dilution Linearity	82 - 120%
Recovery Rate	79 - 108%
Intra-assay Variability	4.5%
Inter-assay Variability	6.4%

 

Components

Component Number	Component Name	Storage Temperature	P162007S	P162007E
P162007-A	ELISA Plate	2~8℃	48 T	96 T
P162007-B	Standard	2~8℃	1 tube	2 tubes
P162007-C	Detection Antibody	2~8℃	120 μL	240 μL
P162007-D	Enzyme Conjugate	2~8℃(Avoid Light)	30 μL	60 μL
P162007-E	Sample Dilution Buffer	2~8℃	8 mL	15 mL
P162007-F	Antibody/Enzyme Dilution Buffer	2~8℃	15 mL	30 mL
P162007-G	20x Wash Buffer	2~8℃	25 mL	50 mL
P162007-H	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162007-I	Stop Solution	Room Temperature	5 mL	10 mL
P162007-J	Plate Sealant Film	Room Temperature	3 pieces	5 pieces

 

Storage

The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded after use; they should not be reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After Initial Use

Component Name	Storage Conditions
ELISA Plate	Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
Detection Antibody	Use within 48 hours after dilution,store at 2~8°C to avoid contamination.
Enzyme Conjugate
Sample Dilution Buffer	Store at 2~8°C for 1 month,avoiding contamination.
Antibody/Enzyme Dilution Buffer
20x Wash Buffer
Substrate Solution	Store at 2~8°C for 1 month,avoiding light exposure.
Stop Solution	Can be stored at room temperature.
Plate Sealant Film