Product description

Human CXCL10 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human CXCL10 (Interferon-γ-induced Protein 10) in serum, plasma, and cell culture supernatants. Specific antibodies against Human CXCL10 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the CXCL10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody (biotin-labeled) is added and incubated to bind. Following another wash, an enzyme conjugate (HRP-labeled Streptavidin) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of CXCL10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm (primary wavelength) and 630 nm (secondary wavelength).

IP-10 was initially identified as an IFN-γ-induced gene in monocytes, fibroblasts, and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 determines its membership in the CXC chemokine subfamily.

 

Specifications

Item Number	P162021S / P162021E
Specification	48 T / 96 T
Detection Range	15.63-1000 pg/mL
Detection Method	Sandwich ELISA
Detection Species	Human
Detection Time	4.75 hours
Sensitivity	4.460 pg/mL
Dilution Linearity	72.2 - 119.4%
Recovery Rate	73.3 - 129.6%
Intra-assay Variability	9.2%
Inter-assay Variability	6.5%

 

Components

Component Number	Component Name	Storage Temperature	P162021S	P162021E
P162021-A	Plate	2~8℃	48 T	96 T
P162021-B	Standard	2~8℃	1 tube	2 tubes
P162021-C	Detection Antibody	2~8℃	1 tube	2 tubes
P162021-D	Enzyme Conjugate	2~8℃(Avoid Light)	150 μL	300 μL
P162021-E	5×Dilution Buffer	2~8℃	12 mL	25 mL
P162021-F	20×Wash Buffer	2~8℃	25 mL	50 mL
P162021-G	Substrate Solution	2~8℃(Avoid Light)	8 mL	15 mL
P162021-H	Stop Solution	Room Temperature	5 mL	10 mL
P162021-I	Plate Sealers	Room Temperature	3 pieces	5 pieces

 

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1. Reagent Storage Table After Initial Use

Material Name	Storage Conditions
Plate	Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
Standard	Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
Detection antibody
Enzyme conjugate	Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
5×Dilution Buffer	Store at 2~8°C for 1 month, avoiding contamination.
20×Wash Buffer
Substrate solution	Store at 2~8°C for 1 month, avoiding light exposure.
Stop solution	Can be stored at room temperature.
Plate Sealers